Journal: PLoS ONE

Article Title: Chemical proteasome inhibition as a novel animal model of inner retinal degeneration in rats

doi: 10.1371/journal.pone.0217945

Figure Lengend Snippet: MG-262 (closed columns) was intravitreally injected at the dose of 0.1 (nmol/eye). For the control group (open column), vehicle (10% DMSO in distilled water) was injected. One and three days following intravitreal injection, the retina was isolated and poly-ubiquitinated protein levels in each retinal lysate were determined by ELISA. The retinal poly-ubiquitinated protein level was normalized to a total protein content in each retinal lysate. Each value represents the mean ± S.E.M. of 4 eyes from 2 animals. ***P<0.001, compared with vehicle by Dunnett’s multiple comparison test.

Article Snippet: Poly-ubiquitinated protein contents were determined by a solid phase sandwich ELISA using anti-poly-ubiquitin monoclonal antibody according to the manufacturer’s instruction manual (CycLex, Nagano, Japan).

Techniques: Injection, Control, Isolation, Enzyme-linked Immunosorbent Assay, Comparison

Journal: Cells

Article Title: Induced Pluripotent Stem Cell-Derived Neural Precursors Improve Memory, Synaptic and Pathological Abnormalities in a Mouse Model of Alzheimer’s Disease

doi: 10.3390/cells10071802

Figure Lengend Snippet: 3xTg-AD mice transplanted with iPSC-NPCs displayed lower levels of ThS-positive amyloid deposits and soluble and insoluble Aβ in brains. ( a – d ) ThS-stained brain slices from 3xTg-AD iPSC-NPCs and PBS-injected mice were analyzed with an epifluorescent microscope. ThS-positive fibrillar deposits was reduced in the hippocampus of 3xTg-AD iPSC-NPCs-injected mice ( a , c ) compared with PBS-injected mice ( b , d ). Scale bar: ( a , b ) 300 μm; ( c , d ) 25 μm. ( e ) The burden of ThS-positive deposits was attenuated in 3xTg-AD iPSC-NPCs mice compared to 3xTg-AD PBS mice (student t -test, ** p < 0.01). ( f ) The quantity of PBS soluble and formic acid (FA) insoluble (aggregated) total Aβ was elevated in 3xTg-AD PBS mice compared to the iPSC-NPCs-injected counterpart as measured by ELISA ( n = 7–8/group) (student t -test, ** p < 0.01; * p < 0.05). Data corresponds to means ± SEM.

Article Snippet: All resulting samples were stored at −80 °C until measured by a solid phase sandwich ELISA for the determination of a total human Aβ (1-x) (IBL America, Spring lake Park, MN, USA).

Techniques: Staining, Injection, Microscopy, Enzyme-linked Immunosorbent Assay